• Pagefile.sys
• *.pst
• %systemroot%\System32\Spool (replace %systemroot% with actual directory)
• %systemroot%\SoftwareDistribution\Datastore (replace %systemroot% with actual directory)
• %allusersprofile%\NTUser.pol
• %Systemroot%\system32\GroupPolicy\registry.pol

• Drive:\Program Files\Call Manager
• Drive:\Program Files\Call Manager Serviceability
• Drive:\Program Files\Call Manager Attendant

On Citrix systems, the following extensions have been causing performance problems. Exclude these file extensions to avoid any performance problems: *.LOG, *.DAT, *.TMP, *.POL, *.PF.

The data directory is used to store Domino email messages. Repeated scanning of this folder while it is being updated with new messages is not an efficient way to scan locally stored email. Use virus scanning applications such as ScanMail for Domino to handle email viruses. By default, the Domino data directory for a non-partitioned installation is <drive>: \ Lotus \ Domino \ Data.

• <drive>: \ EXCHSRVR \ MDBData
• <drive>: \ EXCHSRVR \ MTAData
• <drive>: \ EXCHSRVR \ Mailroot
• <drive>: \ EXCHSRVR \ SrsData
• <drive>: \ WINNT \ system32 \ InetSrv

• <drive>: \ EXCHSRVR \ MDBData
• <drive>: \ EXCHSRVR \ MTAData
• <drive>: \ EXCHSRVR \ Mailroot
• <drive>: \ EXCHSRVR \ SrsData
• <drive>: \ WINNT \ system32 \ InetSrv
• <drive>: \ EXCHSRVR \ MdbDataUtility

Refer to this Microsoft article: File-Level Antivirus Scanning on Exchange 2010

• <drive>: \ EXCHSRVR \ IMCData
• <drive>: \ EXCHSRVR \ MDBData

To configure the scan exclusions for G-Buster Anti-Fraud Syste, refer to the knowledge base article: Setting scan exclusons for G-Buster Anti-Fraud System in Worry-Free Business Security (WFBS). 

This option is best disabled. If it is enabled, it may create unnecessary network traffic when the end users access remote paths or mapped network drives. It can severely impact the user’s experience. Consider disabling this function if all workstations have OfficeScan client installed and are updated to the latest virus signature.

• <drive>: \ WINNT \ SYSVOL
• <drive>: \ WINNT \ NTDS
• <drive>: \ WINNT \ ntfrs
• <drive>: \ WINNT \ system32 \ dhcp
• <drive>: \ WINNT \ system32 \ dns

Exclude the directory or partition where MS Exchange stores its mailbox. Use virus scanning applications like ScanMail for Exchange to handle email viruses. Installable File System (IFS) drive M must also be excluded to prevent the corruption of the Exchange Information Store.

Web Server log files should be excluded from scanning. By default, IIS logs are saved in <drive>:\inetpub\logs\.

Web Server log files should be excluded from scanning. By default, IIS logs are saved in:

• <drive>: \ WINNT \ system32 \ LogFiles
• <drive>: \ WINNT \ system32 \ IIS Temporary Compressed Files

• <drive>: \ Program Files \ Microsoft ISA Server \ ISALogs
• <drive>: \ Program Files \ Microsoft SQL Server \ MSSQL$MSFW \ Data

• <drive>: \ Documents and Settings \ All Users \ Application Data \ Microsoft \ Microsoft Operations Manager
• <drive>: \ Program Files \ Microsoft Operations Manager 2005

• <drive>: \ Program Files \ SharePoint Portal Server
• <drive>: \ Program Files \ Common Files \ Microsoft Shared \ Web Storage System
• <drive>: \ Windows \ Temp \ Frontpagetempdir
• M:\

• Drive:\Program Files\Common Files\Microsoft Shared\Web Server Extensions
• Drive:\Windows\Microsoft.NET\Framework64\v2.0.50727\Temporary ASP.NET Files
• Drive:\Users\ServiceAccount\AppData\Local\Temp
• Drive:\Users\Default\AppData\Local\Temp
• Drive:\Users\<the account that the search service is running as>\AppData\Local\Temp
• Drive:\WINDOWS\system32\LogFiles
• Drive:\Windows\Syswow64\LogFiles

Because scanning may hinder performance, large databases should not be scanned. Since Microsoft SQL Server databases are dynamic, they exclude the directory and backup folders from the scan list. If it is necessary to scan database files, a scheduled task can be created to scan them during off-peak hours.

• <drive>:\ WINNT \ Cluster (if using SQL Clustering)
• <drive>: \ Program Files \ Microsoft SQL Server \ MSSQL \ Data
• Q:\ (if using SQL Clustering)
• C:\Program Files\Microsoft SQL Server\MSSQL.X\OLAP\Data
• File extensions to exclude: .mdf, .ldf, .ndf, .bak, .tm

SQL Server 2012

• %ProgramFiles%\Microsoft SQL Server\MSSQL11.<Instance Name>\MSSQL\Binn\SQLServr.exe
• %ProgramFiles%\Microsoft SQL Server\MSRS11.<Instance Name>\Reporting Services\ReportServer\Bin\ReportingServicesService.exe
• %ProgramFiles%\Microsoft SQL Server\MSAS11.<Instance Name>\OLAP\Bin\MSMDSrv.exe

SQL Server 2008 R2

• %ProgramFiles%\Microsoft SQL Server\MSSQL10_50.<Instance Name>\MSSQL\Binn\SQLServr.exe
• %ProgramFiles%\Microsoft SQL Server\MSSQL10_50.<Instance Name>\Reporting Services\ReportServer\Bin\ReportingServicesService.exe
• %ProgramFiles%\Microsoft SQL Server\MSSQL10_50.<Instance Name>\OLAP\Bin\MSMDSrv.exe

SQL Server 2008

• %ProgramFiles%\Microsoft SQL Server\MSSQL10.<Instance Name>\MSSQL\Binn\SQLServr.exe
• %ProgramFiles%\Microsoft SQL Server\MSSQL10.<Instance Name>\Reporting Services\ReportServer\Bin\ReportingServicesService.exe
• %ProgramFiles%\Microsoft SQL Server\MSSQL10.<Instance Name>\OLAP\Bin\MSMDSrv.exe

SQL Server 2005

• %ProgramFiles%\Microsoft SQL Server\MSSQL.1\MSSQL\Binn\SQLServr.exe
• %ProgramFiles%\Microsoft SQL Server\MSSQL.3\Reporting Services\ReportServer\Bin\ReportingServicesService.exe
• %ProgramFiles%\Microsoft SQL Server\MSSQL.2\OLAP\Bin\MSMDSrv.exe

Considerations for clustering

You can run antivirus software on a SQL Server cluster. However, you must make sure that the antivirus software is a cluster-aware version. Contact your antivirus vendor about cluster-aware versions and interoperability.

If you are running antivirus software on a cluster, make sure that you also exclude these locations from virus scanning:

• Q:\ (Quorum drive)
• C:\Windows\Cluster

• SMS \ Inboxes \ SMS_Executive Thread Name
• SMS_CCM \ ServiceData

• <drive:>\ WSUS
• <drive:>\ WsusDatabase

• MySQL main directory - <Drive>:\mysql\
• MySQL Temporary Files - Uses the Windows system default, which is usually C:\windows\temp\

• SAP ABAP or Java installs:

  \usr\sap\ 

• SAP Content Server Install:

  \SAPDB\

• SAP Printer Server:

  SAPSprint.exe

• Servers where are SAPGui is installed:

  lsagent.exe

• During SAP installs or upgrades, it is recommended to exclude the base SAPinst directories and subdirectories:

  ..\Program Files\SAPinst_instdir\

• C:\Program Files\Novell\Zenworks
• Exclude the following files: NalView.exe, RMenf.exe, ZenNotifyIcon.exe, ZenUserDaemon.exe, casa.msi, dluenf.dll, fileInfo.db, lcredmgr.dll, objInfo.db
• Exclude the following extensions: .APPSTATE, .LOG, .TMP, .ZC

• .dbf - Database file
• .log - Online Redo Log
• .rdo - Online Redo Log
• .arc - Archive log
• .ctl - Control files

Make sure the checkbox for "Do not scan the directories where Trend Micro products are installed." is enabled in WFBS’s Exclusion List settings (Security Settings>Antivirus/Anti-spyware>Exclusions).

Other file extension types that should be added to the exclusion list include large flat and designed files, such as VMWare disk partition. Scanning VMWare partitions while attempting to access them can affect session loading performance and the ability to interact with the virtual machine. Exclusions can be configured for the directory(ies) that contain the Virtual Machines, or by excluding *.vmdk and *.vmem files.

Backup process takes longer to finish when real-time scan is enabled. There are also instances when real-time scan detects an infected file in the volume shadow copy but cannot enforce the scan action because volume shadow copies have read-only access.
It is also advisable to apply the latest Microsoft patches for the Volume Shadow Copies service. Refer to this Microsoft article: A Volume Shadow Copy Service (VSS) update package is available for Windows Server 2003.